Assembly of a processive messenger RNA polyadenylation complex.
نویسندگان
چکیده
منابع مشابه
Aberrant Herpesvirus-Induced Polyadenylation Correlates With Cellular Messenger RNA Destruction
Regulation of messenger RNA (mRNA) stability plays critical roles in controlling gene expression, ensuring transcript fidelity, and allowing cells to respond to environmental cues. Unregulated enhancement of mRNA turnover could therefore dampen cellular responses to such signals. Indeed, several herpesviruses instigate widespread destruction of cellular mRNAs to block host gene expression and e...
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Obtaining pure mRNA preparations from prokaryotes has been difficult, if not impossible, for want of a poly(A) tail on these messages. We have used poly(A) polymerase from yeast to effect specific polyadenylation of Escherichia coli polysomal mRNA in the presence of magnesium and manganese. The polyadenylated total mRNA, which could be subsequently purified by binding to and elution from oligo(...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 1993
ISSN: 0261-4189
DOI: 10.1002/j.1460-2075.1993.tb05690.x